Using nanoparticles for in situ vaccination against cancer: mechanisms and immunotherapy benefits

Using nanoparticles for in situ vaccination against cancer: mechanisms and immunotherapy benefits

Immunotherapy to deal with most cancers is now a longtime scientific strategy. Immunotherapy may be utilized systemically, as accomplished with checkpoint blockade antibodies, but it surely can be injected straight into recognized tumors, in a method of in situ vaccination (ISV). ISV is designed to stimulate a robust native antitumor immune response involving each innate and adaptive immune cells, and via this generate a systemic antitumor immune response against metastatic tumors. Quite a lot of ISVs have been utilized to generate an immunostimulatory tumor microenvironment (TME).

These embody attenuated microorganisms, recombinant proteins, small molecules, bodily disruptors of TME (alternating magnetic and centered ultrasound heating, photothermal remedy, and radiotherapy), and extra not too long ago nanoparticles (NPs). NPs are engaging and distinctive since they will load a number of medicine or different reagents to affect immune and most cancers cell capabilities in the TME, affording a novel alternative to stimulate antitumor immunity. Here, we describe the NP-ISV therapeutic mechanisms, assessment chemically synthesized NPs (i.e., liposomes, polymeric, chitosan-based, inorganic NPs, and so forth.), biologically derived NPs (virus and bacteria-based NPs), and energy-activated NP-ISVs in the context of their use as native ISV. Data means that NP-ISVs can improve outcomes of immunotherapeutic regimens together with these using tumor hyperthermia and checkpoint blockade therapies.

The complement system is a conserved element of innate immunity that fulfills numerous roles in protection and homeostasis. Inappropriate activation of complement contributes to many inflammatory illnesses, nonetheless, which has led to a renewed emphasis on improvement of therapeutic complement inhibitors. Activation of complement element C3 is required for amplification of complement and is achieved via two multisubunit proteases known as C3 convertases. Of these, the choice pathway (AP) C3 convertase is accountable for a majority of the C3 activation merchandise in vivo, which renders it a gorgeous goal for inhibitor discovery.

In this research, we report the identification and characterization of two associated sluggish off-rate modified DNA aptamers (SOMAmer) reagents that inhibit formation of the AP C3 convertase by binding to the proprotease, issue B (FB). These aptamers, often known as SL1102 (31 bases) and SL1103 (29 bases), comprise uniform substitutions of 5-(N-2-naphthylethylcarboxyamide)-2′-deoxyuridine for deoxythymidine. SL1102 and SL1103 bind FB with Ok d values of 49 and 88 pM, respectively, and inhibit activation of C3 and lysis of rabbit erythrocytes beneath AP-specific circumstances.

Cocrystal buildings of SL1102 (3.four Å) and SL1103 (3.1 Å) certain to human FB revealed that SL1102 and SL1103 acknowledge a web site on the juncture of the CCP1, CCP3, and vWF domains of FB. Consistent with these buildings and beforehand revealed data, these aptamers inhibited FB binding to C3b and blocked formation of the AP C3 convertase. Together, these outcomes show potent AP inhibition by modified DNA aptamers and increase the pipeline of FB-binding molecules with favorable pharmacologic properties.

Higher-Order Structure Characterization of NKG2A/CD94 Protein Complex and Anti-NKG2A Antibody Binding Epitopes by Mass Spectrometry-Based Protein Footprinting Strategies

NK group 2 member A (NKG2A), an immune checkpoint inhibitor, is an rising therapeutic goal in immuno-oncology. NKG2A kinds a heterodimer with CD94 on the cell floor of NK and a subset of T cells and acknowledges the nonclassical human leukocyte antigen (HLA-E) in people. Therapeutic blocking antibodies that block the ligation between HLA-E and NKG2A/CD94 have been proven to boost antitumor immunity in mice and people. In this research, we illustrate the sensible utilities of mass spectrometry (MS)-based protein footprinting in areas from reagent characterization to antibody epitope mapping.

Hydrogen/deuterium trade mass spectrometry (HDX-MS) in the higher-order construction characterization of NKG2A in complicated with CD94 offers novel insights into the conformational dynamics of NKG2A/CD94 heterodimer. To absolutely perceive antibody/goal interactions, we employed complementary protein footprinting strategies, together with HDX-MS and quick photochemical oxidation of proteins (FPOP)-MS, to find out the binding epitopes of therapeutic monoclonal antibodies focusing on NKG2A. Such a mix strategy offers molecular insights into the binding mechanisms of antibodies to NKG2A with excessive specificity, demonstrating the blockade of NKG2A/HLA-E interplay.

 Using nanoparticles for in situ vaccination against cancer: mechanisms and immunotherapy benefits

Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling

The discovery of induced pluripotent stem cells (iPSCs) permits for institution of human embryonic stem-like cells from numerous grownup human somatic cells (e.g., fibroblasts), with out the necessity for destruction of human embryos. This offers an unprecedented alternative the place patient-specific iPSCs may be subsequently differentiated to many cell varieties, e.g., cardiac cells and neurons, in order that we will use these iPSC-derived cells to check patient-specific illness mechanisms and conduct drug testing and screening. Critically, these cells have limitless therapeutic potentials, and there are lots of ongoing scientific trials to analyze the regenerative potentials of those iPSC-derivatives in people. However, the standard iPSC reprogramming strategies have drawback of insertional mutagenesis due to use of the integrating viral vectors.

Recombinant Human Transcription Factor-A

7-06637 1µg Ask for price

Recombinant Human Transcription Factor-A

7-06638 5µg Ask for price

Recombinant Human Transcription Factor-A

7-06639 50µg Ask for price

Human Tumor Immunity Primer Library

HTIMU-I 1 set
EUR 548

Mouse Tumor Immunity Primer Library

MTIMU-I 1 set
EUR 548

Recombinant Human Epidermal Growth Factor (a. a. 53)

DAG300 0.5mg
EUR 762

IRGM Immunity-Related GTPase Family, M Human Recombinant Protein

PROTA1A4Y4 Regular: 20ug
EUR 317
Description: IRGM Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 180 amino acids (23-181) and having a molecular mass of 20.1 kDa. IRGM is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Recombinant Transcription Factor A, Mitochondrial (TFAM)

4-RPH050Hu01
  • EUR 508.58
  • EUR 239.00
  • EUR 1632.16
  • EUR 610.72
  • EUR 1121.44
  • EUR 403.00
  • EUR 3930.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q00059
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 51.4kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Transcription Factor A, Mitochondrial expressed in: E.coli

Recombinant Transcription Factor A, Mitochondrial (TFAM)

4-RPH050Mu01
  • EUR 583.84
  • EUR 259.00
  • EUR 1914.40
  • EUR 704.80
  • EUR 1309.60
  • EUR 454.00
  • EUR 4636.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P40630
  • Buffer composition: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 53.6kDa
  • Isoelectric Point: 9.1
Description: Recombinant Mouse Transcription Factor A, Mitochondrial expressed in: E.coli

Recombinant Transcription Factor A, Mitochondrial (TFAM)

4-RPH050Ra01
  • EUR 592.80
  • EUR 262.00
  • EUR 1948.00
  • EUR 716.00
  • EUR 1332.00
  • EUR 460.00
  • EUR 4720.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q91ZW1
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 50.4kDa
  • Isoelectric Point: Inquire
Description: Recombinant Rat Transcription Factor A, Mitochondrial expressed in: E.coli

Recombinant Nuclear Factor I/A (NFIA)

4-RPC655Hu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q12857
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 20.8kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Nuclear Factor I/A expressed in: E.coli

Immunity Related GTPase M (IRGM) Antibody

abx412110-01mg 0.1 mg
EUR 537
  • Shipped within 1 week.

Recombinant Human Vascular Endothelial Growth Factor (a. a. 165)

DAG304 10ug
EUR 748

Recombinant Escherichia coli Colicin-Ia immunity Protein (aa 1-111)

VAng-Lsx02230-inquire inquire Ask for price
Description: Escherichia coli Colicin-Ia immunity protein, recombinant protein.

Recombinant Escherichia coli Colicin-Ia immunity Protein (aa 1-32)

VAng-Lsx02231-1mgEcoli 1 mg (E. coli)
EUR 2938
Description: Escherichia coli Colicin-Ia immunity protein (aa 1-32), recombinant protein.

Recombinant Escherichia coli Colicin-Ia immunity Protein (aa 1-32)

VAng-Lsx02231-500gEcoli 500 µg (E. coli)
EUR 2223
Description: Escherichia coli Colicin-Ia immunity protein (aa 1-32), recombinant protein.

Recombinant Escherichia coli Colicin-Ib immunity Protein (aa 1-115)

VAng-Lsx02232-inquire inquire Ask for price
Description: Escherichia coli Colicin-Ib immunity protein, recombinant protein.

Goat Anti-Human recombinant Fetuin A (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG

FETB12-A 100 ug
EUR 482

Goat Anti-Human Apolipoprotein A-IV A(ApoA4) peptid IgG, aff pure

APOA45-A 100 ul
EUR 482

Rabbit Anti-human MART-1/Melanocyte Differentiation-A/Melan-A peptide (CT) IgG

MART12-A 100 ul
EUR 445

Recombinant Human Fibroblast Growth Factor 2 (Basic) (a. a. 155)

DAG302 50ug
EUR 748

PDGF-A Platelet-Derived Growth Factor A Human Recombinant Protein

PROTP04085-2 Regular: 20ug
EUR 317
Description: Platelet-Derived Growth Factor A Human Recombinant short chain produced in E.Coli is a non-glycosylated, polypeptide chain containing 110 amino acids fragment (87-196) and having a total Mw of 17.02kDa, with an amino-terminal hexahistidine tag. ;PDGF-A is purified by proprietary chromatographic techniques.

HGF-A Hepatocyte Growth Factor A Chain Human Recombinant Protein

PROTP14210-4 Regular: 10ug
EUR 317
Description: HGF-A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 463 amino acids fragment (32-494) having a total molecular weight of 57.8kDa. The HGF-A is fused with a 4.5kDa amino-terminal hexahistidine tag.;The HGF-A is purified by proprietary chromatographic techniques.

Mating Factor a

H-7724.0001 1.0mg
EUR 96
Description: Sum Formula: C82H114N20O17S; CAS# [59401-28-4] net

Mating Factor a

H-2925.0001 1.0mg
EUR 126
Description: Sum Formula: C82H114N20O17S; CAS# [59401-28-4] net

Mating Factor a

H-2925.0005 5.0mg
EUR 418
Description: Sum Formula: C82H114N20O17S; CAS# [59401-28-4] net

Boc-a-Me-DL-Val-OH

A-4145.0001 1.0g
EUR 139
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4]

Boc-a-Me-DL-Val-OH

A-4145.0005 5.0g
EUR 477
Description: Sum Formula: C11H21NO4; CAS# [139938-00-4]

Recombinant Human Platelet Derived Growth Factor-A

7-01351 5µg Ask for price

Recombinant Human Platelet Derived Growth Factor-A

7-01352 20µg Ask for price

Recombinant Human Platelet Derived Growth Factor-A

7-01353 1mg Ask for price

Recombinant Human Hepatocyte Growth Factor A Chain

7-00631 2µg Ask for price

Recombinant Human Hepatocyte Growth Factor A Chain

7-00632 10µg Ask for price

Recombinant Human Hepatocyte Growth Factor A Chain

7-00633 1mg Ask for price

Recombinant Human Platelet Derived Growth Factor-A

CG093-005 5ug
EUR 293

Recombinant Human Platelet Derived Growth Factor-A

CG093-020 20ug
EUR 408

Recombinant Human Platelet Derived Growth Factor-A

CG093-101 1mg
EUR 7475

Recombinant Nuclear Prelamin A Recognition Factor (NARF)

4-RPC651Hu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q9UHQ1
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 18.0kDa
  • Isoelectric Point: 9.3
Description: Recombinant Human Nuclear Prelamin A Recognition Factor expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Bo01
  • EUR 565.92
  • EUR 254.00
  • EUR 1847.20
  • EUR 682.40
  • EUR 1264.80
  • EUR 442.00
  • EUR 4468.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P15691
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.8kDa
  • Isoelectric Point: 7.6
Description: Recombinant Bovine Vascular Endothelial Growth Factor A expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Ca01
  • EUR 637.60
  • EUR 274.00
  • EUR 2116.00
  • EUR 772.00
  • EUR 1444.00
  • EUR 490.00
  • EUR 5140.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q9MYV3
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 19.1kDa
  • Isoelectric Point: Inquire
Description: Recombinant Dog Vascular Endothelial Growth Factor A expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Hu01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P15692
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 20.6kDa
  • Isoelectric Point: 6.9
Description: Recombinant Human Vascular Endothelial Growth Factor A expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Mu01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q00731
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.8kDa
  • Isoelectric Point: 6.5
Description: Recombinant Mouse Vascular Endothelial Growth Factor A expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Po01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P49151
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 51.2kDa
  • Isoelectric Point: 6.5
Description: Recombinant Pig Vascular Endothelial Growth Factor A expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Ra01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P16612
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 20.8kDa
  • Isoelectric Point: 7.5
Description: Recombinant Rat Vascular Endothelial Growth Factor A expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Rb01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q866G4
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.9kDa
  • Isoelectric Point: 6.2
Description: Recombinant Rabbit Vascular Endothelial Growth Factor A expressed in: E.coli

Recombinant Vascular Endothelial Growth Factor A (VEGFA)

4-RPA143Si01
  • EUR 574.88
  • EUR 257.00
  • EUR 1880.80
  • EUR 693.60
  • EUR 1287.20
  • EUR 448.00
  • EUR 4552.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 25.1kDa
  • Isoelectric Point: 9.2
Description: Recombinant Simian Vascular Endothelial Growth Factor A expressed in: E.coli

TFAM Transcription Factor-A Recombinant Protein Human

PROTQ00059 Regular: 5ug
EUR 317
Description: TFAM produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (43-246.a.a) and having a molecular mass of 26.6kDa. ;TFAM is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Rabbit Anti-Influenza A HA (H1N1, 1-343aa) (A/Wyoming/3/03) protein IgG, aff pure

H3N22-A 100 ul
EUR 445

Fmoc-a-amino-DL-Gly(Boc)-OH

A-4320.0001 1.0g
EUR 273
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7]

Fmoc-a-amino-DL-Gly(Boc)-OH

A-4320.0005 5.0g
EUR 998
Description: Sum Formula: C22H24N2O6; CAS# [176039-39-7]

Chicken Anti-Protein A IgG, aff pure

PRTA11-A 1 mg
EUR 482

Goat Anti-Protein-A IgG aff pure

PRTA12-A 0.1 ml
EUR 408

Chicken Anti-Protein-A IgG aff pure

PRTA13-A 100 ug
EUR 408

Rabbit Anti-Influenza A nucleoprotein (H1N1-NP/1-320aa) protein (A/06/California/2009) IgG, aff pure

H1NP21-A 100 ul
EUR 445

Rabbit Anti-Influenza A HA2 (H5N1) (A/Vietnam/1203/2004 366-531aa, >95%, his-tag) protein IgG

HA2H012-A 100 ul
EUR 445

Recombinant Human Insulin Like Growth Factor I (a. a. 70) Polypeptide

DAG296 100ug
EUR 879

Rabbit Anti-Influenza A HA (H3N2, 1-531aa/HA1+HA2) (A/Brisbane/10/2007) protein IgG, aff pure

H3N23-A 100 ul
EUR 445

Goat Anti-Hepatitis A Virus (Strain HM175) IgG

HAV11-A 100 ul
EUR 457

Goat Anti-human Serum Amyloid A (SAA) antiserum

SAA11-A 100 ul
EUR 482

Rabbit Anti-Streptococcus A IgG, aff pure, #2

STA12-A 1 mg
EUR 286

Recombinant Human Immunity-related GTPase family M protein Protein, GST, E.coli-100ug

QP6236-ec-100ug 100ug
EUR 408

Recombinant Human Immunity-related GTPase family M protein Protein, GST, E.coli-10ug

QP6236-ec-10ug 10ug
EUR 200

Recombinant Human Immunity-related GTPase family M protein Protein, GST, E.coli-1mg

QP6236-ec-1mg 1mg
EUR 1632

Recombinant Human Immunity-related GTPase family M protein Protein, GST, E.coli-200ug

QP6236-ec-200ug 200ug
EUR 634

Recombinant Human Immunity-related GTPase family M protein Protein, GST, E.coli-500ug

QP6236-ec-500ug 500ug
EUR 1060

Recombinant Human Immunity-related GTPase family M protein Protein, GST, E.coli-50ug

QP6236-ec-50ug 50ug
EUR 263

Recombinant Escherichia coli Colicin-E5 immunity protein in ColE9 (aa 1-83)

VAng-Lsx02228-1mgEcoli 1 mg (E. coli)
EUR 2759
Description: Escherichia coli Colicin-E5 immunity protein in ColE9, recombinant protein.

Recombinant Escherichia coli Colicin-E5 immunity protein in ColE9 (aa 1-83)

VAng-Lsx02228-500gEcoli 500 µg (E. coli)
EUR 1961
Description: Escherichia coli Colicin-E5 immunity protein in ColE9, recombinant protein.

Recombinant Escherichia coli Colicin-E5 immunity protein in ColE9 (aa 1-83)

VAng-Lsx02228-50gEcoli 50 µg (E. coli)
EUR 1356
Description: Escherichia coli Colicin-E5 immunity protein in ColE9, recombinant protein.

Recombinant Rat Tumor Necrosis Factor alpha (TNF-a)

DAG287 20ug
EUR 983

Recombinant human Transcription elongation factor A protein 3

P2240 100ug Ask for price
  • Uniprot ID: O75764
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Transcription elongation factor A protein 3

Recombinant human Chromatin assembly factor 1 subunit A

P2784 100ug Ask for price
  • Uniprot ID: Q13111
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Chromatin assembly factor 1 subunit A

Recombinant Platelet Derived Growth Factor Subunit A (PDGFA)

4-RPA528Ra01
  • EUR 404.64
  • EUR 211.00
  • EUR 1242.40
  • EUR 480.80
  • EUR 861.60
  • EUR 334.00
  • EUR 2956.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 15.2kDa
  • Isoelectric Point: 9.1
Description: Recombinant Rat Platelet Derived Growth Factor Subunit A expressed in: E.coli

Immunity-Related GTPase Family M Protein (IRGM) Antibody

20-abx126951
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody

abx026104-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody

abx026104-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family Q Protein (IRGQ) Antibody

abx032348-400ul 400 ul
EUR 551
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family Q Protein (IRGQ) Antibody

abx032348-80l 80 µl
EUR 321
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody

20-abx321743
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family M Protein (IRGM) Protein

20-abx260986
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody

20-abx339551
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody

abx448534-100ug 100 ug
EUR 523
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family Q Protein (IRGQ) Antibody

abx234399-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody

20-abx210849
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Human Immunity-related GTPase family M protein (IRGM)

1-CSB-EP011827HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 47.1 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Immunity-related GTPase family M protein(IRGM) expressed in E.coli

Rabbit Anti-Human Calcineurin A-subunit IgG aff pure

CALNA12-A 100 ug
EUR 482

Chicken Anti-Human+Mouse IgG+A+M IgG/Y

HGAM11-A 100 ul
EUR 482

Fmoc-Mating Factor a

B-1620.0001 1.0mg
EUR 126
Description: Sum Formula: C97H124N20O19S; CAS# [250642-63-8]

Fmoc-Mating Factor a

B-1620.0005 5.0mg
EUR 418
Description: Sum Formula: C97H124N20O19S; CAS# [250642-63-8]

Factor XIII A Antibody

20-abx009275
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Fmoc-Mating Factor a

5-01154 4 x 1mg Ask for price

Hematoxylin, Weigert's Iron (Part A)

HWI-A-250 250 ml
EUR 116

Recombinant Human Colony Stimulating Factor 1 (a. a. 2 x 159), Polypeptide

DAG292 25ug
EUR 971

Rabbit Anti-Human Apolipoprotein A-I protein IgG, aff pure

APOA12-A 100 ug
EUR 482

Rabbit Anti-Mouse Apolipoprotein A-I protein IgG, aff pure

APOA13-A 100 ug
EUR 482

Goat Anti-Human Apolipoprotein A-Il protein IgG, aff pure

APOA21-A 100 ug
EUR 482

Goat Anti-Green Fluorescent Proteins (GFP, A. victoria) protein, IgG

GFP11-A 100 ul
EUR 408

Rabbit Anti-Mouse a-1-Acid Glycoprotein IgG, aff pure

A1AG18-A 100 ug
EUR 482

Rabbit Anti-Rat a-1-Acid Glycoprotein IgG, aff pure

A1AG19-A 100 ug
EUR 482

Rabbit Anti-Mouse prepro Orexin A IgG # 1, aff pure

PPOX11-A 100 ul
EUR 482

Rabbit Anti-Rat/Human Orexin-A IgG # 1, aff pure

OXA11-A 100 ug
EUR 482

Goat Anti-lethal factor (B Anthracis) IgG

LF16-A 100 ug
EUR 408

Rabbit Anti-Influenza A hemeagglutinin A2 (HA2/stem or stalk domain/347-523aa) protein (A/Viet Nam/1203/2004(H5N1) IgG, aff pure

HA28-A 100 ul
EUR 445

Rabbit Anti-Hemagglutinin HA1 Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) IgG

H11N2-01-A 100 ul
EUR 482

Rabbit Anti-bovine Calcineurin (A+B subunits) protein, IgG, aff pure

CALN11-A 100 ug
EUR 482

Rabbit Anti-Borrelia burgdorferi Osp-A protein (OspA) IgG, aff pure

OSPA12-A 100 ul
EUR 482

Goat Anti-Anthrax Edema Factor (B Anthracis) IgG

EF11-A 100 ug
EUR 408

Sheep Anti-Human Prothrombin (Factor II) IgG, Purified

PRTN11-A 1 mg
EUR 225

Sheep Anti-Mouse Prothrombin (Factor II) IgG, Purified

PRTN13-A 100ul
EUR 482

Recombinant Human Coagulation Factor XIII A Chain (C-6His)

C880-10ug 10ug
EUR 156
Description: Supplied as a 0.2 μm filtered solution of 50 mM NaCl,5% Sucrose, 1% Tween 20 (v/v),0.3% Histidine (w/v),pH8.0.

Recombinant Human Coagulation Factor XIII A Chain (C-6His)

C880-1mg 1mg
EUR 1877
Description: Supplied as a 0.2 μm filtered solution of 50 mM NaCl,5% Sucrose, 1% Tween 20 (v/v),0.3% Histidine (w/v),pH8.0.

Recombinant Human Coagulation Factor XIII A Chain (C-6His)

C880-500ug 500ug
EUR 1328
Description: Supplied as a 0.2 μm filtered solution of 50 mM NaCl,5% Sucrose, 1% Tween 20 (v/v),0.3% Histidine (w/v),pH8.0.

Recombinant Human Coagulation Factor XIII A Chain (C-6His)

C880-50ug 50ug
EUR 369
Description: Supplied as a 0.2 μm filtered solution of 50 mM NaCl,5% Sucrose, 1% Tween 20 (v/v),0.3% Histidine (w/v),pH8.0.

Recombinant Human Transcription Elongation Factor A (SII)-Like 1

7-06619 5µg Ask for price

Recombinant Human Transcription Elongation Factor A (SII)-Like 1

7-06620 20µg Ask for price

Recombinant Human Transcription Elongation Factor A (SII)-Like 1

7-06621 1mg Ask for price

TNF-a Tumor Necrosis Factor-Alpha Rabbit Recombinant Protein

PROTP04924 Regular: 20ug
EUR 317
Description: Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E.Coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. ;The TNF-alpha is purified by standard chromatographic techniques.

TNF-a Tumor Necrosis Factor-Alpha Mouse Recombinant Protein

PROTP06804 Regular: 20ug
EUR 317
Description: Tumor Necrosis Factor-a Mouse Recombinant produced in E. coli is a single, non glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17301.32 Dalton. ;The TNF-alpha is purified by standard chromatographic techniques.

TNF-a Tumor Necrosis Factor-Alpha Rat Recombinant Protein

PROTP16599 Regular: 20ug
EUR 317
Description: Tumor Necrosis Factor-a Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17339.44 Dalton. The TNF-alpha is purified by standard chromatographic techniques.

TNF-a Tumor Necrosis Factor-Alpha Porcine Recombinant Protein

PROTP23563 Regular: 20ug
EUR 317
Description: Tumor Necrosis Factor-a Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17,274 Dalton. The TNF-alpha is purified by standard chromatographic techniques.

Recombinant human Pre-mRNA-processing factor 40 homolog A

P2804 100ug Ask for price
  • Uniprot ID: O75400
  • Reconstitution: Metal affinity chromatography on Fn Super Capacity Column (Nickel)
Description: Recombinant protein for human Pre-mRNA-processing factor 40 homolog A

TNF-a Tumor Necrosis Factor-Alpha Canine Recombinant Protein

PROTP51742 Regular: 20ug
EUR 317
Description: TNF-a Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 17.3 kDa. ;The TNF-a is purified by proprietary chromatographic techniques.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (ALP)

abx448005-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (APC)

abx448006-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (Biotin)

abx448007-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (FITC)

abx448008-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (HRP)

abx448009-100ug 100 ug
EUR 565
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (PerCP)

abx448011-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (RPE)

abx448012-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Immunity-Related GTPase Family M Protein (IRGM) Antibody (Streptavidin)

abx448013-100ug 100 ug
EUR 578
  • Shipped within 5-12 working days.

Rabbit Anti-Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) IgG

H1N1-01-A 100 ul
EUR 482

Goat Anti-Human/mouse/rat Insulin chain A peptide IgG, aff pure

INSA21-A 100 ul
EUR 482

Rabbit Anti-Rat neurite outgrowth inhibitor (Nogo-A) IgG #1, aff. pure

NogoA11-A 100 ug
EUR 482

Vascular Endothelial Growth Factor A-A (vegfaa) Antibody

20-abx320017
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Rabbit Anti-Rat Epithelial Na Channel alpha (ENAC-a) IgG #1, Aff. Pure

ENACa11-A 100 ug
EUR 482

While numerous advances have been made to mitigate this difficulty, together with the usage of chemical substances, excisable and non-integrating vectors, and use of the modified mRNA, security stays a priority. Both integrating and non-integrating strategies additionally endure from many different limitations together with low effectivity, variability, and tumorigenicity.

The non-integrating mRNA reprogramming is of excessive effectivity, however it’s delicate to reagents and want approaches to cut back the immunogenic response. An various non-integrating and safer approach of producing iPSCs is through direct supply of recombinant cell-penetrating reprogramming proteins into the cells to be reprogrammed, however reprogramming effectivity of the protein-based strategy is extraordinarily low in comparison with the traditional virus-based nuclear reprogramming. Herein, we describe detailed steps for environment friendly era of human iPSCs by protein-based reprogramming in mixture with stimulation of the Toll-like receptor 3 (TLR3) innate immune pathway.