Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity.

Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is to pseudotype biosafety-level-2 viral particles with Spike. Such pseudotyping has now been described for single-cycle lentiviral, retroviral, and vesicular stomatitis virus (VSV) particles, but the reagents and protocols are not widely available.

anti-CD26 Activated T, B lymphocytes and macrophages
MBS485039-5x1mg 5x1mg
EUR 5795

anti-CD26 Activated T, B lymphocytes and macrophages
512-A-01 100 µg Ask for price

anti-CD26 Activated T, B lymphocytes and macrophages
512-A-02 1 mg Ask for price

Activated Macrophages rat monoclonal antibody, clone 88a, Aff - Purified
AM26130PU-N 100 µg Ask for price

Macrophages (PE)
MBS6251992-01mL 0.1(mL
EUR 875

Macrophages (PE)
MBS6251992-5x01mL 5x0.1mL
EUR 3790

Macrophages (AP)
MBS6248110-01mL 0.1(mL
EUR 875

Macrophages (AP)
MBS6248110-5x01mL 5x0.1mL
EUR 3790

Macrophages (HRP)
MBS6251045-01mL 0.1(mL
EUR 875

Macrophages (HRP)
MBS6251045-5x01mL 5x0.1mL
EUR 3790

Macrophages (APC)
MBS6268962-01mL 0.1mL
EUR 875

Macrophages (APC)
MBS6268962-5x01mL 5x0.1mL
EUR 3790

Macrophages (FITC)
MBS6250036-01mL 0.1(mL
EUR 875

Macrophages (FITC)
MBS6250036-5x01mL 5x0.1mL
EUR 3790

Macrophages (Biotin)
MBS6249123-01mL 0.1(mL
EUR 875

Macrophages (Biotin)
MBS6249123-5x01mL 5x0.1mL
EUR 3790

Macrophages Antibody
GWB-DAD90D 0.1 mg Ask for price

MACROPHAGES, Antibody
GWB-DE8FCB 0.1 mg Ask for price

Macrophages Antibody
GWB-B68A2C 2 ml Ask for price

Macrophages Antibody
GWB-3243CF 0.2 ml Ask for price

Macrophages Antibody
GWB-33EA8D 0.2 mg Ask for price

Macrophages Antibody
GWB-36F900 0.2 ml Ask for price

Macrophages Antibody
GWB-45D900 0.02 mg Ask for price

Macrophages, Monocytes
MBS6507914-01mg 0.1mg
EUR 765

Macrophages, Monocytes
MBS6507914-5x01mg 5x0.1mg
EUR 3300

Monocytes, Macrophages
MBS6507971-01mg 0.1mg
EUR 730

Monocytes, Macrophages
MBS6507971-5x01mg 5x0.1mg
EUR 3140

Macrophage, 86kD (Mature Macrophages)
MBS633054-01mg 0.1mg
EUR 940

Macrophage, 86kD (Mature Macrophages)
MBS633054-5x01mg 5x0.1mg
EUR 4075

Macrophages (MaxLight 405)
MBS6253019-01mL 0.1(mL
EUR 875

Macrophages (MaxLight 405)
MBS6253019-5x01mL 5x0.1mL
EUR 3790

Macrophages (MaxLight 490)
MBS6254059-01mL 0.1(mL
EUR 875

Macrophages (MaxLight 490)
MBS6254059-5x01mL 5x0.1mL
EUR 3790

Macrophages (MaxLight 550)
MBS6255099-01mL 0.1(mL
EUR 875

Macrophages (MaxLight 550)
MBS6255099-5x01mL 5x0.1mL
EUR 3790

Macrophages (MaxLight 650)
MBS6256139-01mL 0.1(mL
EUR 875

Macrophages (MaxLight 650)
MBS6256139-5x01mL 5x0.1mL
EUR 3790

Macrophages (MaxLight 750)
MBS6257178-01mL 0.1mL
EUR 875

Macrophages (MaxLight 750)
MBS6257178-5x01mL 5x0.1mL
EUR 3790

Macrophages antibody (PE)
MBS832895-01mg 0.1mg
EUR 700

Macrophages antibody (PE)
MBS832895-5x01mg 5x0.1mg
EUR 3010

Macrophages antibody (PE)
61R-1268 100 ug Ask for price

Rat Alveolar Macrophages
ABC-TC5506 1 vial Ask for price
Description: Rat Priamry Alveolar Macrophages are derived from the lung of 6-8 weeks old Sprague–Dawley Rats. Each vial contains at least 0.5x106 cells. Rat Alveolar Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

Macrophages Antibody (RPE)
GWB-Q01144 100 TESTS Ask for price

Monocytes, Macrophages (PE)
MBS6252045-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (PE)
MBS6252045-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (PE)
MBS6252046-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (PE)
MBS6252046-5x01mL 5x0.1mL
EUR 4365

Monocytes, Macrophages (AP)
MBS6248165-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (AP)
MBS6248165-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (AP)
MBS6248166-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (AP)
MBS6248166-5x01mL 5x0.1mL
EUR 4365

Macrophages, Monocytes (PE)
MBS6244404-01mL 0.1(mL
EUR 1055

Macrophages, Monocytes (PE)
MBS6244404-5x01mL 5x0.1mL
EUR 4600

Monocytes, Macrophages (PE)
MBS6244411-01mL 0.1(mL
EUR 1020

Monocytes, Macrophages (PE)
MBS6244411-5x01mL 5x0.1mL
EUR 4440

Macrophages, Monocytes (AP)
MBS6241740-01mL 0.1(mL
EUR 1055

Macrophages, Monocytes (AP)
MBS6241740-5x01mL 5x0.1mL
EUR 4600

Monocytes, Macrophages (AP)
MBS6241747-01mL 0.1(mL
EUR 1020

Monocytes, Macrophages (AP)
MBS6241747-5x01mL 5x0.1mL
EUR 4440

Anti-Mouse Macrophages
RM924920 0.1 mg Ask for price

Macrophages Antibody (FITC)
GWB-665EFD 0.02 mg Ask for price

Macrophages Antibody (FITC)
GWB-4562DA 0.1 mg Ask for price

Monocytes, Macrophages (HRP)
MBS6251100-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (HRP)
MBS6251100-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (HRP)
MBS6251101-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (HRP)
MBS6251101-5x01mL 5x0.1mL
EUR 4365

Macrophages, Monocytes (HRP)
MBS6243871-01mL 0.1(mL
EUR 1055

Macrophages, Monocytes (HRP)
MBS6243871-5x01mL 5x0.1mL
EUR 4600

Monocytes, Macrophages (HRP)
MBS6243878-01mL 0.1(mL
EUR 1020

Monocytes, Macrophages (HRP)
MBS6243878-5x01mL 5x0.1mL
EUR 4440

Macrophages, Monocytes (APC)
MBS6242273-01mL 0.1(mL
EUR 1055

Macrophages, Monocytes (APC)
MBS6242273-5x01mL 5x0.1mL
EUR 4600

Monocytes, Macrophages (APC)
MBS6242280-01mL 0.1(mL
EUR 1020

Monocytes, Macrophages (APC)
MBS6242280-5x01mL 5x0.1mL
EUR 4440

Monocytes, Macrophages (APC)
MBS6269017-01mL 0.1mL
EUR 1105

Monocytes, Macrophages (APC)
MBS6269017-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (APC)
MBS6269018-01mL 0.1mL
EUR 1005

Monocytes/Macrophages (APC)
MBS6269018-5x01mL 5x0.1mL
EUR 4365

Monocytes, Macrophages (FITC)
MBS6250085-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (FITC)
MBS6250085-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (FITC)
MBS6250086-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (FITC)
MBS6250086-5x01mL 5x0.1mL
EUR 4365

Macrophages, Monocytes (FITC)
MBS6243338-01mL 0.1(mL
EUR 1055

Macrophages, Monocytes (FITC)
MBS6243338-5x01mL 5x0.1mL
EUR 4600

Monocytes, Macrophages (FITC)
MBS6243345-01mL 0.1(mL
EUR 1020

Monocytes, Macrophages (FITC)
MBS6243345-5x01mL 5x0.1mL
EUR 4440

MATURE MACROPHAGES, Antibody
GWB-8E4353 0.05 mg Ask for price

MATURE MACROPHAGES, Antibody
GWB-ED7BB8 0.1 mg Ask for price

Monocytes, Macrophages (Biotin)
MBS6249176-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (Biotin)
MBS6249176-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (Biotin)
MBS6249177-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (Biotin)
MBS6249177-5x01mL 5x0.1mL
EUR 4365

Macrophages, Monocytes (Biotin)
MBS6242805-01mL 0.1(mL
EUR 1055

Macrophages, Monocytes (Biotin)
MBS6242805-5x01mL 5x0.1mL
EUR 4600

Monocytes, Macrophages (Biotin)
MBS6242812-01mL 0.1(mL
EUR 1020

Monocytes, Macrophages (Biotin)
MBS6242812-5x01mL 5x0.1mL
EUR 4440

MOUSE ANTI PIG MACROPHAGES
MBS224390-01mg 0.1mg
EUR 380

MOUSE ANTI PIG MACROPHAGES
MBS224390-5x01mg 5x0.1mg
EUR 1545

CD68 (KP1) Macrophages, human
MBS397062-05mL 0.5mL
EUR 735

CD68 (KP1) Macrophages, human
MBS397062-5x05mL 5x0.5mL
EUR 3155

Rat Bone Marrow Macrophages
ABC-TC4086 1 vial Ask for price
Description: Rat bone marrow macrophages (RPBMMACs) are derived from the tibias and femurs of Sprague–Dawley rats. RPBMMACs are grown in 100 mm treated tissue culture dish and incubated in Culture Complete Growth Medium for 10-15 days. Cultures are then expanded.

MOUSE ANTI HUMAN MACROPHAGES
MBS216638-002mg 0.02mg
EUR 210

MOUSE ANTI HUMAN MACROPHAGES
MBS216638-5x002mg 5x0.02mg
EUR 765

MOUSE ANTI HUMAN MACROPHAGES
MBS213180-02mg 0.2mg
EUR 500

MOUSE ANTI HUMAN MACROPHAGES
MBS213180-5x02mg 5x0.2mg
EUR 2070

MOUSE ANTI HUMAN MACROPHAGES
MBS213181-01mg 0.1mg
EUR 355

MOUSE ANTI HUMAN MACROPHAGES
MBS213181-5x01mg 5x0.1mg
EUR 1425

Rat Pan Macrophages antibody
MBS531965-01mg 0.1mg
EUR 1805

Rat Pan Macrophages antibody
MBS531965-5x01mg 5x0.1mg
EUR 7975

Rat Pan Macrophages antibody
10R-M157a 100 ug Ask for price

Pig Macrophages Antibody (FITC)
GWB-Q01229 0.1 mg Ask for price

Rat macrophages (CD163), Antibody
GWB-5ACF20 0.25 mg Ask for price

Mouse Pan Macrophages antibody
MBS532409-02mg 0.2mg
EUR 2455

Mouse Pan Macrophages antibody
MBS532409-5x02mg 5x0.2mg
EUR 10885

Mouse Pan Macrophages antibody
MBS530836-02mg 0.2mg
EUR 1805

Mouse Pan Macrophages antibody
MBS530836-5x02mg 5x0.2mg
EUR 7975

Mouse Pan Macrophages antibody
10R-M156b 100 ug Ask for price

Mouse Pan Macrophages antibody
10R-M156a 200 ug Ask for price

Guinea Pig Macrophages Antibody
GWB-Q00951 2 ml Ask for price

Rat Hepatic Primary Macrophages
CC4F163 5x 10^5 Cells/Vial Ask for price

Human iPSC-derived macrophages
CSC-00834L One Frozen vial Ask for price

Mouse Thymic Primary Macrophages
CC4F186 5x 10^5 Cells/Vial Ask for price

Anti-Mouse Macrophages Antibody
103-M101 100 µg
EUR 399
Description: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with isolated mouse macrophages).

Monocytes, Macrophages (MaxLight 405)
MBS6253074-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (MaxLight 405)
MBS6253074-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (MaxLight 405)
MBS6253075-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (MaxLight 405)
MBS6253075-5x01mL 5x0.1mL
EUR 4365

Monocytes, Macrophages (MaxLight 490)
MBS6254114-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (MaxLight 490)
MBS6254114-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (MaxLight 490)
MBS6254115-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (MaxLight 490)
MBS6254115-5x01mL 5x0.1mL
EUR 4365

Monocytes, Macrophages (MaxLight 550)
MBS6255154-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (MaxLight 550)
MBS6255154-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (MaxLight 550)
MBS6255155-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (MaxLight 550)
MBS6255155-5x01mL 5x0.1mL
EUR 4365

Monocytes, Macrophages (MaxLight 650)
MBS6256194-01mL 0.1(mL
EUR 1105

Monocytes, Macrophages (MaxLight 650)
MBS6256194-5x01mL 5x0.1mL
EUR 4815

Monocytes/Macrophages (MaxLight 650)
MBS6256195-01mL 0.1(mL
EUR 1005

Monocytes/Macrophages (MaxLight 650)
MBS6256195-5x01mL 5x0.1mL
EUR 4365

Macrophages, Monocytes (MaxLight 490)
MBS6245470-01mL 0.1(mL
EUR 1055

Macrophages, Monocytes (MaxLight 490)
MBS6245470-5x01mL 5x0.1mL
EUR 4600

Monocytes, Macrophages (MaxLight 490)
MBS6245477-01mL 0.1(mL
EUR 1020

Here, we detailed how to effectively pseudotype lentiviral particles with SARS-CoV-2 Spike and infect 293T cells engineered to express the SARS-CoV-2 receptor, ACE2. We also made all the key experimental reagents available in the BEI Resources repository of ATCC and the NIH.

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