Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity.

Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is to pseudotype biosafety-level-2 viral particles with Spike. Such pseudotyping has now been described for single-cycle lentiviral, retroviral, and vesicular stomatitis virus (VSV) particles, but the reagents and protocols are not widely available.

Anti-Macrophages antibody

STJ16100760 100 µg
EUR 354.00

Anti-Macrophages antibody

STJ16100868 200 µg
EUR 814.00

Mouse Pan Macrophages antibody

10R-M156a 200 ug
EUR 975.00
Description: Rat monoclonal Mouse Pan Macrophages antibody

Mouse Pan Macrophages antibody

10R-M156b 100 ug
EUR 705.00
Description: Rat monoclonal Mouse Pan Macrophages antibody

Rat Pan Macrophages antibody

10R-M157a 100 ug
EUR 835.00
Description: Mouse monoclonal Rat Pan Macrophages antibody

Hcn2 ELISA Kit| Rat Potassium/sodium hyperpolarization-activate

EF018832 96 Tests
EUR 689.00

Hcn4 ELISA Kit| Rat Potassium/sodium hyperpolarization-activate

EF018833 96 Tests
EUR 689.00

Mouse Anti Human Macrophages Monoclonal Antibody

DMABT-49597MH 0.2 ml
EUR 403.00

Mouse Anti Human Macrophages Monoclonal Antibody

DMABT-49598MH 2 ml
EUR 840.00

anti-CD26 T/B lymphocytes and macrophages

512-A-01mg 0,1 mg
EUR 267.50
  • Category: Antibody, Signal Transduction Antibodies, mAb
Description: anti-CD26 Activated T, B lymphocytes and macrophages

anti-CD26 T/B lymphocytes and macrophages

512-A-1000ug 1000 ug
EUR 1282.50
  • Category: Antibody, Signal Transduction Antibodies, mAb
Description: anti-CD26 Activated T, B lymphocytes and macrophages

Mouse Anti Guinea Pig Macrophages Monoclonal Antibody

DMABT-51930MG 2 ml
EUR 767.00

Mouse Anti Pig Macrophages Monoclonal Antibody,FITC

DMABT-52020MP 0.1 mg
EUR 881.00

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody

DMABT-54496RM 25 µg
EUR 372.00

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody

DMABT-54497RM 0.25 mg
EUR 767.00

Rat Anti Human Macrophages And Neutrophils Monoclonal Antibody

DMABT-49599RH 200 TEST
EUR 767.00

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,FITC

DMABT-54493RM 25 µg
EUR 445.00

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,FITC

DMABT-54494RM 50 µg
EUR 663.00

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,FITC

DMABT-54495RM 0.1 mg
EUR 741.00

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,RPE

DMABT-54498RM 25 TEST
EUR 429.00

Rat Anti Mouse Macrophages/Monocytes Monoclonal Antibody,RPE

DMABT-54499RM 100 TEST
EUR 663.00

Here, we detailed how to effectively pseudotype lentiviral particles with SARS-CoV-2 Spike and infect 293T cells engineered to express the SARS-CoV-2 receptor, ACE2. We also made all the key experimental reagents available in the BEI Resources repository of ATCC and the NIH.